CATALYST
Concatenation
Normalization
Compensation
Debarcoding
NOTE: THIS WEB APP IS NOT ACTIVELY MAINTAINED PLEASE USE THE BIOCONDUCTOR R PACKAGE
We designed CATALYST (Cytometry dATa anALYSis Tools) to provide tools for preprocessing of cytometry data, including i) concatenation, ii) normalization using bead standards, iii) single-cell deconvolution, and iv) bead-based compensation.
The preprint to this project is accesible on
bioRxiv
at
Chevrier et al., 2017:
Channel crosstalk correction in suspension and imaging mass cytometry.
Our
Cell Systems
paper is available at
Chevrier et al., 2017:
Compensation of Signal Spillover in Suspension and Imaging Mass Cytometry.
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Yield plot
Spillover matrix
Before vs. after scatters
Use pre-acquired spillover matrix
Estimate spill from controls
Upload multiplexed data (FCS)
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Select method:
Flow compensation
NNLS compensation
Compensate
Yield plot
Event plot
Mahal plot
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